rat mouse elisa kits  (Boster Bio)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Caffeamide 36-13 Regulates the Antidiabetic and Hypolipidemic Signs of High-Fat-Fed Mice on Glucose Transporter 4, AMPK Phosphorylation, and Regulated Hepatic Glucose Production

doi: 10.1155/2014/821569

Figure Lengend Snippet: Primers used in this study.

Article Snippet: The levels of adiponectin, leptin, and insulin in blood were analyzed by ELISA using a commercial assay kit according to manufacturer's directions (mouse/rat adiponectin ELISA kit, B-Bridge International, GmbH, Germany; mouse insulin ELISA kit, Shibayagi, Gunma, Japan; and mouse leptin ELISA kit, Morinaga, Yokohama, Japan).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Caffeamide 36-13 Regulates the Antidiabetic and Hypolipidemic Signs of High-Fat-Fed Mice on Glucose Transporter 4, AMPK Phosphorylation, and Regulated Hepatic Glucose Production

doi: 10.1155/2014/821569

Figure Lengend Snippet: Effects of caffeamide 36-13 (TS) on absolute tissue weight, food intake, liver lipids and blood profiles.

Article Snippet: The levels of adiponectin, leptin, and insulin in blood were analyzed by ELISA using a commercial assay kit according to manufacturer's directions (mouse/rat adiponectin ELISA kit, B-Bridge International, GmbH, Germany; mouse insulin ELISA kit, Shibayagi, Gunma, Japan; and mouse leptin ELISA kit, Morinaga, Yokohama, Japan).

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Caffeamide 36-13 Regulates the Antidiabetic and Hypolipidemic Signs of High-Fat-Fed Mice on Glucose Transporter 4, AMPK Phosphorylation, and Regulated Hepatic Glucose Production

doi: 10.1155/2014/821569

Figure Lengend Snippet: Semiquantitative RT-PCR analysis on PEPCK, G6Pase, SREBP1c, FAS, apo C-III, and adiponectin mRNA expression in liver tissue of the mice by oral gavage caffeamide 36-13 (TS) for 4 weeks. All values are means ± S.E. ( n = 9). ## P < 0.001, ### P < 0.001 compared with the control (CON) group; * P < 0.05, ** P < 0.01, *** P < 0.001 compared with the high-fat + vehicle (HF) group. TS: T1: 10, T2: 20 mg/kg bodyweight; Rosi: rosiglitazone (0.01 g/kg body weight). Total RNA (1 μ g) isolated from tissue was reverse transcripted by MMLV-RT, and 10 μ L of RT products were used as templates for PCR. Signals were quantitated by image analysis; each value was normalized by GAPDH.

Article Snippet: The levels of adiponectin, leptin, and insulin in blood were analyzed by ELISA using a commercial assay kit according to manufacturer's directions (mouse/rat adiponectin ELISA kit, B-Bridge International, GmbH, Germany; mouse insulin ELISA kit, Shibayagi, Gunma, Japan; and mouse leptin ELISA kit, Morinaga, Yokohama, Japan).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Isolation